assuming your datatable variable is called dt
a. use add data column activity to add data column called Col6 (if it doesnt exist)
b. use invoke code
click edit arguments and add in/out argument dt
then click edit code and paste this
dt.AsEnumerable().ToList().ForEach(Sub(row) row("Col6")= row("Col1").ToString+row("Col2").ToString+row("Col4").ToString+row("Col5").ToString)
this will set Col6 = col1+col2+col4+col5
