Use Read Range for the existing excel.
Add DataColumn with Column Name as ID
then use the below query
DataTableVariable2 = (From x In DataTableVariable1.AsEnumerable()
Select DataTableVariable1.Clone.Rows.Add({x(“Account”),convert.ToString(x(“Account”)).split("-"c)(1).Substring(0,3)})).copytodatatable
you can use DataTableVariable2 as u require