Unchecking "Allow unlisted file name extensions" results in 404.7 error

Can we uncheck the setting "Allow unlisted file name extensions" in IIS ?

Setting “Allow unlisted file name extensions” is needed for Orchestrator as the pages are dynamically generated and they don’t have extensions; or the pages have extensions that are uniquely generated each time and look like a GUID (Example: 193031F0-D938-4108-B9CC-4ABD39C25FBC). In other words, “Allow unlisted file name extensions” need to be always checked. 

To find this setting, open IIS and select Orchestrator website. Double-click on “Request Filtering” and select “File Name Extensions” tab. Now click on “Edit Feature Settings”. Below is a screenshot for reference.